The effects of sub-lethal doses of copper sulfate in zebra fish ovary tissue

Abstract

Introduction: Copper is one of the heavy metals used in aquaculture for the elimination of algae, as well as prevention and treatment of fish diseases. The effects of sub-lethal doses of copper sulfate in zebra fish ovary tissue were investigated in 30-day exposure.
Materials & Methods: For this purpose, 270 females with average weight of 0.3±0.05 were subjected to 0, 0.02 and 0.04 mg/l cuso4. At the end trail, samplings were done from gonad tissue and the tissue sections were prepared.
Result: Results showed that in the control group, ovary tissue was normal and major of the oocytes were sexually at the beginning and end of fourth stage. In 0.02 mg/l oocytes were in the second and third stage. In 0.04 mg/l, the slight growth of ovarian was observed and oocytes were in the second stages and a number of oocytes were observed at the beginning of third stage and atrotic follicles were observed in gonad tissue.
Conclusion: This process shows an increase in egg growth with increasing copper sulfate concentration.