Effects of different levels of sodium alginate with low molecular weight on some blood factors and expression of immune-related genes in the common carp (Cyprinus carpio)

Effects of different levels of sodium alginate with low molecular weight on some blood factors and expression of immune-related genes in the common carp (Cyprinus carpio)

Authors

  • Monire Sahli Department of Fisheries, Faculty of Fisheries and Environment, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran, Iran, Islamic Republic of
  • Hamed Paknezhad Department of Fisheries, Faculty of Fisheries and Environment, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran, Iran, Islamic Republic of
  • Seyed Hossein Hoseinifar Department of Fisheries, Faculty of Fisheries and Environment, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran, Iran, Islamic Republic of
  • Mohammad Sudagar Department of Fisheries, Faculty of Fisheries and Environment, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran, Iran, Islamic Republic of
  • Mohammad Mazandarani Department of Fisheries, Faculty of Fisheries and Environment, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran, Iran, Islamic Republic of

Keywords:

Interleukin-6 (IL-6) Sodium alginate Gene expression Blood factor Tumor necrosis factor (TNF-1α) Common carp

Abstract

Introduction: This study aimed to investigate the effects of using different levels of low molecular weight sodium alginate on blood factors and immune-related expression genes in common carp (Cyprinus carpio).
Materials & Methods: For this purpose, 240 carp fry with an average weight of about 19.12±0.40 g were prepared and stored in 12 200-liter tanks (4 treatments and each treatment in 3 replications). After a two-week adaptation period, juveniles with diets containing 0 (control group; diet without sodium alginate), 5, 10, and 20 g of sodium alginate per kg of food for seven weeks (daily 3% of fish body weight and fed twice a day). At the end of the experiment, some blood factors and expression of TNF-1α and IL-6 genes were measured.
Results: There was no significant difference in blood factors (red blood cell count, hematocrit, hemoglobin, MCV, MCH and MCHC) (P<0.05), But a significant increase in white blood cell count was observed in the treatment of 0.5% sodium alginate compared to the control group (P<0.05). However, differential white blood cell counts (lymphocytes, monocytes, neutrophils and eosinophils) were not significant (P<0.05). There was a significant difference in the relative expression of TNF-1α gene (P<0.05). In treatments containing 1% and 2% sodium alginate, a decrease in relative expression of TNF-1α gene was observed (P<0.05), But in the
treatment containing 0.5% sodium alginate, no significant difference was observed with the control group (P<0.05). Also, a significant increase in relative expression of IL-6 gene was observed in the treatment containing 1% sodium alginate compared to the control group (P<0.05). In the treatment containing 2% sodium alginate, the relative expression of IL-6 gene was significantly reduced (P<0.05), But in the treatment containing 0.5% sodium alginate, no significant difference was observed with the control group (P<0.05).
Conclusion: The results showed that the use of sodium alginate in common carp is effective as a stimulant of the immune system.

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Published

2023-02-08

Issue

Vol. 15 No. 1 (2023)

Section

Articles

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