Assessment of genetic structure of common buzzard species in East Azerbaijan using PCR-RAPD Markers

Abstract

Introduction: The discussion over the conservation of the genetic resources of wild species is a hot topic in scientific circles around the world. The objective of the present study was the identification of genetic diversity of captive Buzzard one of the predatory birds in East Azerbaijan province using PCR-RAPD markers.
Materials & Methods: For this purpose, In the first step, blood sampling was performed from the wing vein of 10 birds and DNA extraction and subsequently 15 arbitrary primers were applied for PCR-RAPD technique.
Result: After electrophoresis, selected primers exhibited sufficient variability, which yielded overall 150 distinct bands, 29 polymorphic bands, for details, range of a number of bands per primer was 11 to 22, and product size varied between 234 to 1230 bp. Highest and lowest polymorphic primers were OPA-14, OPA-13, respectively as a general conclusion, analyses using more regions, more birds, and more DNA markers will be useful to confirm or to reject these findings.
Conclusion: However, the management implications and decisions regarding the genetic conservation of this bird require further sampling from multiple populations.