Identification and isolation of nitrogen-fixing bacteria in rainbow trout farms in Firoozkooh city

Abstract

Introduction: In this study, in order to identification and isolation of nitrogen-fixing
bacteria in rainbow trout breeding ponds, sixty fish breeding ponds and four sampling
stations in Firoozkooh city were selected.
Materials & Methods: After taking a certain volume of pool water in the place of biofilters,
their culture is optimized in Nutrition agar and LB-Broth medium and incubated at 25 to 30
degrees for 5 to 6 days. The final culture in Nitrosamines and culture medium Nitrobacteria
was performed and the grown colonies were identified.
Results: The results showed that the highest number of isolated bacterial colonies (more
than 300 colonies) were in the biofilter input and the lowest amount of nitrification bacterial
colonies in the culture medium was related to the water well inlet culture (3 colonies).
Conclusion: The results of this study showed that the activity of fish farming can change
the values of water parameters used during salmon farming and cause some undesirable
physicochemical factors of the water used. The extent of this effect is largely due to
environmental conditions to the process of fish farming and the intensity of nitrification
bacteria in the pool water.