Improving the production of vitamin B12 by Streptomyces griseus under different growth conditions

Abstract

Vitamin B12 (cobalamin) is a cobalt-comprising corrinoid important for human metabolism. Its compound construction contains cobalt organized through a corrin ring, a benzimidazole nitrogen, and numerous ligands. Microbial fermentation, principally via Streptomyces types, is a crucial manufacturing foundation of Vitamin B12. This research aimed to improve Vitamin B12 creation through two isolates of Streptomyces griseus by diverse media, extraction techniques, and growth situations, as well as to assess the outcome of architecturally linked composites on Vitamin B12 production. Mutually isolates stood cultured in two different media, and Vitamin B12 stayed extracted by four recognized ways, comprising those of McCormick & Wright (1971) and Florent & Ninet (1979). The more effective isolate (IN) stayed re-propagated below variable pH standards and temperatures to conclude optimum creation circumstances. Moreover, seven Vitamin B12 similarities stood individually additional to the medium to evaluate their consequence on produce. The medium comprising CoCl₂·6H₂O sustained the uppermost Vitamin B12 creation. The (IN) isolate produced 123.0 μg/ml under optimal conditions: inoculum density of 2.4×10⁶ spores/ml, incubation at 28 °C, and pH 8.0, yielding up to 130.9 μg/ml. Among the additives, 2′, 2′-Bipyridine-3′, 3′-diol enhanced production to 149.5 μg/ml, while other compounds inhibited growth. Vitamin B12 was primarily cell-bound (39.02 μg/ml) rather than secreted (6.3 μg/ml). Optimal medium composition, pH, temperature, and precursor supplementation significantly enhance Vitamin B12 biosynthesis by S. griseus. Further studies are recommended to refine these parameters for industrial-scale production