Effect of different levels of selenium nanoparticles on some sperm quality indices of rainbow trout (Oncorhynchus mykiss)

Abstract

Introduction: In this study, the effects of dietary selenium nanoparticles on sperm quality
and reproductive performance of male rainbow trout (Oncorhynchus mykiss) breeders
were investigated.
Materials & Methods: A total of 120 male breeding fish were selected from the breeding
herd of Shahid Motahari Genetic Research and Breeding Fish Center of Shahid Motahari
in Yasouj. After adaptation, fish were divided into 4 experimental groups with 3
replications. Male breeders fed commercial feed (no selenium nanoparticles) (control
group) and male breeders fed a diet containing 0.5, 1 and 2 mg selenium nanoparticles per
kg diet. After qualitative evaluation of sperm, proliferation and fertilization operations
were performed for different groups using control ova.
Results: The results showed that the highest sperm volume and the highest sperm density
were observed in rainbow trout breeders fed with 2 mg selenium nanoparticles per kg diet.
The lowest sperm volume was observed in the control groups and 0.5 mg selenium
nanoparticles and the lowest sperm density was observed in the control group. Sperm
motility time in fish fed diets containing selenium nanoparticles (0.5, 1 and 2 mg) was
significantly longer than the control group fed diets containing selenium nanoparticles.
There was no significant difference between the percentage of motile spermatozoa and
spermatocytes between the experimental groups.
Conclusion: In general, dietary selenium nanoparticles improve sperm quality in male
rainbow trout breeders.