Evaluation of blood biochemical parameters and tissue damage of gill and liver of rainbow trout in acetaminophen

Abstract

Introduction: The aim of this study was to evaluate the biochemical factors of blood and tissue
damage of gills and liver of rainbow trout in 14-day exposure to acetaminophen.
Materials & Methods: For this purpose, 300 pieces of rainbow trout juveniles with an average
weight (12.52 ± 2.5) were prepared and experimental treatments including 5 treatments and 3
replications were designed.
Results: Based on the obtained results, a significant increase in serum lysozyme and
complement (ACH50) enzyme activity was observed in juvenile fish during exposure to
acetaminophen. The amount of cortisol and glucose also showed a significant decrease in the
amount of these indicators so that the highest amount of cortisol and glucose measured in the
control group and the lowest amount was observed and recorded in treatment 5. The results of
the study of tissue damage showed that in gill tissue, curvature and club formation of the tip of
the secondary gill blade were due to the effects of low concentration (0.01 mg / l) of
acetaminophen. At moderate concentrations (0.1 and 1) mg / l, hyperplasia, hypertrophy,
clubbing of the tip of the secondary gill blade and welding of the secondary blade were
observed. Liver tissue examination showed the most severe liver tissue lesions in fish exposed
to 10 mg / l acetaminophen.
Conclusion: The presence of acetaminophen caused damage to gill tissue and liver in rainbow
trout juveniles. However, it increased blood safety markers such as lysozyme and complement
and also significantly reduced cortisol. Which showed a decrease in stress levels in fish exposed
to different levels of acetaminophen.