Protective Effect of Chitosan-Alginate Nanoparticles Containing Curcumin Against Oxidative Stress Caused by Nickel Oxide Nanoparticles in Carassius auratus

Abstract

Introduction: Metal oxide nanoparticles are among the most widely used
compounds among nanoparticles that have wide applications in various fields.
Nanoparticles have high chemical and biological reactivity by increasing their
surface to volume ratio, which leads to increased production of free radicals. The
generated free radicals disrupt the oxidation state and normal regeneration of body
cells and cause oxidative damage in organisms and many diseases. The aim of this
study was to investigate the oxidant effect of nickel oxide nanoparticles and also the
antioxidant effect of chitosan-alginate nanoparticles in the model alginate model The
fish is goldfish.
Materials & Methods: Accordingly, in this study, 5 groups of fish were used for
experiments with 4-week treatments. The study groups included the control group,
the treatment group with nickel oxide nanoparticles and 3 groups of simultaneous
treatment of nickel oxide nanoparticles and alginate-chitosan nanoparticles
containing curcumin. At the end of the period, liver samples were isolated and
collected for oxidative damage. Total antioxidant levels, MDA, glutathione and the
antioxidant enzymes catalase, glutathione S-transferase and superoxide dismutase
were measured in all groups.
Result: The results showed that treatment with nickel oxide nanoparticles decreased
the level of total antioxidants and increased the level of MDA in the nickel oxide
nanoparticle treatment group, but the groups that received curcumin-containing
alginate-chitosan nanoparticles along with nickel oxide nanoparticles had antioxidant
parameters.
Conclusion: These results showed strong evidence of induction of cellular oxidative
stress due to exposure to nickel oxide nanoparticles as well as the protective effect
of curcumin-containing alginate-chitosan nanoparticles.