Anticancer effects of coral extract of Sinularia compressa on human gastric adenocarcinoma cell line (AGS)

Abstract

Introduction: In recent decades, extensive studies have been conducted on the
effectiveness of marine aquatic extracts in the treatment of diseases including cancer.
Based on the results of various experiments in Southeast Asian countries such as
Thailand and Japan on the effect of the extract on the treatment of various diseases, this
study is an attempt to investigate the effect of coral extract of Sinularia compressa on
human gastric adenocarcinoma (AGS) cells.
Materials & Methods: Different concentrations of coral extract 5, 10, 15, 20, 25, 30,
35, 40, 45, 50 μg/ml were extracted. The AGS cell line was then transferred to the
laboratory under sterile conditions. This cell line was cultured in "DMEM f12" medium.
The cultured cells were then transferred to plate 96 of the well. The cells were incubated
at three different times 18, 24, 48 hours. Then, using MTT and flow cytometers, the
effectiveness of the extract on AGS cells was measured.
Results: Based on the results, it was found that at concentrations above 15 μg/mg, the
cells were isolated from the surface of the plate and suspended in the medium. According
to the results of the MTT method at a concentration of 10 μg/ml, more than 84% of the
cells were destroyed. Based on the findings, the time factor did not show a significant
effect on the results. Factor LC50 showed 50% cell death at a concentration of 7.5 μg/ml
Sinularia coral extract. Due to the high effect of this extract on cancer cell death, the
lowest concentration was tested in flow cytometry. The results of this experiment
showed that cell death of 43% of cells occurred at a concentration of 5 μg / ml of coral
extract, which was consistent with the results of MTT test at this concentration. Cell
shape changes were also examined. Based on morphological observations, the affected
cells became smaller and lost their epithelial shape.
Conclusion: S. compressa coral extract showed a great effect on AGS cell mortality.
These findings confirm that the potential of using bioactive compounds extracted from
marine aquatic animals to control cancer is very significant and can be considered as an
alternative to chemical treatments and high side effects.