The effect of two native probiotics on blood parameters of Sander lucioperca

Abstract

Introduction: The aim of this study was to evaluate the probiotic effects of Enterococcus
faecium and Lactobacillus brevis on blood parameters of Sander lucioperca weighing 14±1 g.
Materials & Methods: The number of fish was 225 completely randomly distributed in 5
treatments with three replications and 15 in each the ponds. Dietary treatments containing
Enterococcus faecium and Lactobacillus brevis (1010, 108 probiotic cells per gram of diet) and
probiotic-free control treatments were fed in three replications for 6 weeks and then evaluated
for blood. Nutritional treatments were named, A2, A1, B1, B2, respectively. A1 (Enterococcus
faecium probiotic (number of bacteria/g 10 10 + basic food), A2 (Enterococcus faecium
probiotic (number of bacteria/g 108 + basic food)), B1 (Lactobacillus brevis probiotic) Number
of bacteria/g 1010
+ Basic food), B2 (probiotic Lactobacillus brevis) number of bacteria/g 1010
+
basic food) and control treatment were Lactobacillus (basic food + physiological serum)
Hematocrit (HCT) was measured using hematocrit capillary tubes and a hematocrit centrifuge.
Hemoglobin by chlorometric method with a wavelength of 540 nm in a spectrophotometer was
measured.
Results: The results showed that HCT increased in A1 treatment compared to control and A2
groups. There were no significant differences in treatments B1 and B2, but they had a significant
increase compared to the control group(P<0.05) Leukocyte count in probiotic treatments was
higher than the control group was significantly higher (P<0.05). Lymphocyte counts in fish fed
diets containing probiotics were significantly higher than in the control group. MCV had a
significant difference in treatments B1 and A1 and control with A2 and B2 (P<0.05). However,
other parameters e.g. RBC, HB, MCH, MCHC had no significant different (P>0.05).
Conclusion: The present study showed that the addition of probiotic bacteria in treatments B1,
A1 to the diet of Sander lucioperca can have a favorable effect on their blood factors.