Evaluation of expression profile of intestinal cells infected with Cryptosporidium parvum using bioinformatics

Abstract

Introduction: Pathogens affect host cellular mechanisms in order to impair the
capabilities of the host cell in the path of infection. Cryptosporidium parvum in people
with AIDS and people with weakness of the immune system creates severe bile and acute
diarrhea, and is known as an important intestinal pathogen in humans and some of the
mammals and rows with Salmonella, Shigella and Enterotoxic genetic strains of
Escherichia coli It takes. From the point of view of cellular genetics, it should be noted
that human intestinal cells due to infection with Cryptosporidium parvum undergo major
changes in host-parasite interactions, especially changes in the expression of relevant
genes. The aim of this study was to identify the effects of altering the expression profile
of genes in HCT-8 cells infected with Cryptosporidium parvum on host-parasite
interaction. In this study, an estimate of the amount of genes involved in biological
processes and in the process of intestinal cell infection with Cryptosporidium parvum was
obtained.
Materials & Methods: In order to carry out the studies, the data recorded in the GEO
database on the NCBI website, which was placed by researchers at the University of
Houston, Texas, USA, were used. Raw data were read, pre-processed and processed using
various software and online databases.
Results: Studies have shown that the expression of genes involved in HCT-8 cell line
infection is affected by Cryptosporidium parvum, and that some genes are over-expressed
and some are under-expressed. The results of this study show that the most genes involved
in biological processes are related to cell-parasite communication and the least genes are
involved in cell death (apoptosis) and DNA replication.
Conclusion: The results of this study show that different genes under the influence of
infection change in expression, each of which can activate different factors and different
pathways in the direction of infection and be considered as future drug and vaccine targets.