Investigation of genetic diversity of Mugger (Crocodylus palustris) in Iran using Tau-Crystallin gene

Abstract

In order to study genetic diversity of Iranian Mugger (Crocodylus palustris), after chosen 5 regions in Sistan and Balochestan province, sampling and DNA extraction from 10 Mugger scab, Tau-Crystallin gene (tissue-type eye lens tau-crystallin) was used. The SSCP technique was used after amplifying of 648 bp in PCR .For observing of SSCP band from PCR product, %8 Polyacrylamide and silver staining were used. In this study, there was 1 allele which showed no fenetic diversity in Tau-Crystallin gene of Iranian Mugger.