Genetic diversity of Oriental sole (Euryglossa orientalis) using microsatellite marker in the Persian Gulf (Khuzestan and Bushehr)

Genetic diversity of Oriental sole (Euryglossa orientalis) using microsatellite marker in the Persian Gulf (Khuzestan and Bushehr)

Authors

  • Maryam Nayerirad Department of Marine Biology, Faculty of Marine Science, Khorramshahr University of Marine Science and Technology, Iran, Iran, Islamic Republic of
  • Hossein Zolgharnean Department of Marine Biology, Faculty of Marine Science, Khorramshahr University of Marine Science and Technology, Iran, Iran, Islamic Republic of
  • Hamid Galledari Department of Genetics, Faculty of Science, Shahid Chamran University, Khuzestan, Iran, Iran, Islamic Republic of
  • Mohammad Ali Salari Aliabadi Department of Marine Biology, Faculty of Marine Science, Khorramshahr University of Marine Science and Technology, Iran, Iran, Islamic Republic of

Keywords:

Genetic diversity, Eurygloss orientalis, microsatellite, Persian Gulf.

Abstract

The aim of this study was to analysis the population genetic structure of Eurygloss orientalis based on microsatellite markers. For these purpose 58 samples of Eurygloss orientalis from two regions of Persian Gulf (Khuzestan and Bushehr) were collected. DNA was extracted and using 6 pairs of microsatellite primers. Polymerase chain reaction (PCR) was conducted out of 6 microsatellite primers and results shows that 2 loci were polymorphic. Analysis revealed that the average number of observed and effective alleles in Khuzestan and Bushehr were 14.5, 10.708 and 13.5, 9.511 respectively. The mean observed and expected heterozygosity was 0.837 and 0.889 respectively. There are no significant deviations from HWE. The Fst value between populations was 0.025. The highest genetic distance between Khuzestan and Bushehr was 0.13 and the lowest genetic similarity was 0.733. The Fst value suggested no genetic differences between populations.

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Published

2013-08-11

Issue

Vol. 5 No. 3 (2013)

Section

Articles

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