The expression of serine protease gene from Virgobacillus natechei based on gene artificial synthesis

Abstract

Introduction: Protein hydrolysis of aquatic wastes is an appropriate and economic strategy
to obtain valuable products. In recent years, with researchers turning their attention to the
use of green and safe technology in the hydrolysis of proteins obtained from aquatic wastes
and choosing a suitable alternative for chemicals, protease enzymes have been introduced to
this industry.
Materials & Methods: In the present study, in order to produce a recombinant enzyme,
after transferring the synthesized gene of serine protease into the vector PET28a, the
structure created in the host E. coli BL21(DE3) was transferred, and the accuracy of the
transfer was checked by performing colony-PCR. Then, the expression of the recombinant
enzyme was performed with a concentration of 1 mM IPTG at a temperature of 28°C for 8
hours.
Results: The result of protein secretion on SDSPAGE was determined by the appearance of
a band with an approximate size of 31 kDa.
Conclusion: Considering the successful production of serine protease from the bacterium
Virgobacillus natechei with the help of artificial synthesis of the gene encoding this enzyme,
we could hope that this recombinant enzyme would be a suitable option for use in various
industries including fishing industry. This would happen if the activity and biochemical
characteristics of the purified enzyme are suitable and additional studies confirm the
possibility of optimal production at a high amount.