A phylogenetic study of Tangsayad and Karayi wild sheep (Ovis orientalis) populations

A phylogenetic study of Tangsayad and Karayi wild sheep (Ovis orientalis) populations

Authors

  • Kavan Nikdel Islamic Azad University, Iran, Iran, Islamic Republic of
  • Siamak Yousefi Islamic Azad University, Iran, Iran, Islamic Republic of
  • Qobad Asgari Jafarabadi Islamic Azad University, Iran, Iran, Islamic Republic of

Keywords:

Wild sheep, Microsatellite markers, Genetic diversity, Heterozygosity, Genetic distance

Abstract

The phylogenetic study of Tangsayad and Kerayi wild sheep populations was conducted by using 5 microsatellite markers (BM6444, MCM63, MCMA2, LSCV38, and OARAE129). The PCR responses for all 5 markers were done truly and each marker was checked. The
OARAE129, LSCV38, MCM63 markers showed a meaningful deviation from Hardy- Weinberg equilibrium in wild sheep populations, and 60% of Poly Morf locus which contains 3 LSCV38, MCM63, OARAE129 markers, and the two MCMA2 and BM6444 markers were
Mono Morf. Low average of Allel for any locus (1.6 Allel) showed the low genetic diversity of wild sheep. Nie (1972, 1978) indicators were used to obtain the genetic distance between two wild sheep populations, which showed the high genetic similarity (0.9615) and (0.9771) and low genetic distance (0.0392) and (0.0232) in Tangsayad and Karayi wild sheep populations. Observing the average heterozygosity showed that life and the future of these animals are in danger.

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Published

2010-04-27

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Section

Articles

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