A histological investigation of macroscopically ovarian maturation in perch (Perca fluviatilis L.) in Anzali wetland

Abstract

Target of the current study was investigation of macroscopic and microscopic (histological) structure of Perch’s ovaries (Perca fluviatiis). To perform this experience, samples were caught monthly from Anzali wetland located in North West of Iran. Ovaries
were harvested and staged macroscopically. Macroscopic observation showed that gonad development in vitellogenic stage was in line with increasing of blood vessels around of ovaries. Also during of vitellogenic stage the mount of pigment was increased and oocytes changed their color from light yellow to orangre and in final maturation tended to be colorless and clear. Ovaries were fixed after dissecting in formalin to be prepared for histological processes. Tissue samples were sectioned 5-7μm, before staining with Mayer’s Haematoxylin and Eosin. Variations of gonad maturation were divided to six stages consisting of previtellogenic, early vitellogenic, late vitellogenic, final maturation, and spent stages. For observation demonstrate a single ovulation and a short time spawning.