Isolation, Cultivation and Characterization of Neural stem cells from Persian Sturgeon (Acipenser persicus) Brain

Isolation, Cultivation and Characterization of Neural stem cells from Persian Sturgeon (Acipenser persicus) Brain

Authors

  • Hadiseh Kashiri Gorgan University of Agricultural Sciences and Natural Resources, Iran, Iran, Islamic Republic of
  • Ali Shabani Gorgan University of Agricultural Sciences and Natural Resources, Iran, Iran, Islamic Republic of
  • Kamran Haidari Gorgan University of Medical Sciences, Iran, Iran, Islamic Republic of
  • Mohammad Reza Imanpour Gorgan University of Agricultural Sciences and Natural Resources, Iran, Iran, Islamic Republic of
  • Mohsen Saeedi Gorgan University of Medical Sciences, Iran, Iran, Islamic Republic of

Keywords:

Antibody, Persian sturgeon, stem cell, culture, brain.

Abstract

In the present study, we tried to cultivate neural stem cells from different regions of Persian sturgeon (Acipenser persicus) brain, as a chondrostei fish. For this, telencephalon, mesencephalon and metencephalon of the brain were separated, cultivated in DMEM/F12
medium supplemented with fetal bovine serum (20% FBS) and maintained at 250C with 5%CO2. Medium was changed every 3s days and replaced with fresh medium. After 2-3 weeks, cells were confluent and passaged. Cells of metencephalon (cerebellum) culture from Persian sturgeon brain (CPS) are still alive after about 7 months and 9 passages and their stocks are available in the laboratory. The cell cultures have been cryopreserved using liquid nitrogen. The survival rate of cells was 70% after thawing. In immonocytochemistry survey, cells were immonopositive for anti-nestin antibody, as a neural stem cell marker. The obtained cultures could be considered as a constant long term culture because of their stability, and could be used in different studies.

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Published

2013-05-06

Issue

Vol. 5 No. 2 (2013)

Section

Articles

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