The process of urbanization is rapidly changing natural habitats, and it is in this way that the behavior of the mammalian creatures has undergone changes due to shifts in the availability of their resources, their habitat, and also as a result of human presence. This paper represents a quantitative assessment of the behavior of mammals on an urban-rural gradient in a combination of camera-trapping, GPS-telemetry, land-use modeling, and activity pattern models. Measurements of diel activity change, home-range compression, vigilance levels, and movement velocities were measured in a limited sample of mammalian species in three urbanizing landscapes. There was also nocturnal activity, decreased movement distance, increased risk-aversion behavior, and reliance on anthropogenic resources as observed in urban areas. Spatial regressions showed that the density of roads, artificial light, and built-up cover were good predictors of the altered behavior. The results demonstrate that behavior-based conservation measures like wildlife corridors, low-light areas, and conflict mitigation systems should be used. The study offers evidence-based behavioral indices to the development of coexistence-based urban planning models and provides a methodological template for quantifying the responses of species to the accelerating urbanization in the developing world.